Carrier for promoting transfection of in-vivo cationic lipid-nucleic acid complex through ultrasonic cavitation as well as preparation method and application of carrier
The invention discloses a carrier for promoting in-vivo cationic lipid-nucleic acid complex transfection through ultrasonic cavitation and a preparation method and application thereof.The carrier comprises microbubbles, a cationic lipid-nucleic acid complex and protein, and the cationic lipid-nuclei...
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| Main Authors | , , , , , |
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| Format | Patent |
| Language | Chinese English |
| Published |
10.05.2024
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The invention discloses a carrier for promoting in-vivo cationic lipid-nucleic acid complex transfection through ultrasonic cavitation and a preparation method and application thereof.The carrier comprises microbubbles, a cationic lipid-nucleic acid complex and protein, and the cationic lipid-nucleic acid complex is adsorbed to the surfaces of the microbubbles; the protein and the cationic lipid-nucleic acid complex are in a combined state through electrostatic adsorption, and under the action of ultrasonic cavitation, the protein and the cationic lipid-nucleic acid complex are transiently separated, so that the transfection performance of the cationic lipid-nucleic acid complex is activated. The carrier provided by the invention is high in stability and low in toxicity after intravenous injection, the cationic lipid-nucleic acid complex and protein can be temporarily removed through ultrasonic cavitation, the difficulty of in-vivo application of the cationic lipid-nucleic acid carrier is overcome, and direct |
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| AbstractList | The invention discloses a carrier for promoting in-vivo cationic lipid-nucleic acid complex transfection through ultrasonic cavitation and a preparation method and application thereof.The carrier comprises microbubbles, a cationic lipid-nucleic acid complex and protein, and the cationic lipid-nucleic acid complex is adsorbed to the surfaces of the microbubbles; the protein and the cationic lipid-nucleic acid complex are in a combined state through electrostatic adsorption, and under the action of ultrasonic cavitation, the protein and the cationic lipid-nucleic acid complex are transiently separated, so that the transfection performance of the cationic lipid-nucleic acid complex is activated. The carrier provided by the invention is high in stability and low in toxicity after intravenous injection, the cationic lipid-nucleic acid complex and protein can be temporarily removed through ultrasonic cavitation, the difficulty of in-vivo application of the cationic lipid-nucleic acid carrier is overcome, and direct |
| Author | LIN TAO QIU CHEN HUANG PINTONG WANG GUOWEI JIN PEILE CHEN JIFAN |
| Author_xml | – fullname: WANG GUOWEI – fullname: QIU CHEN – fullname: JIN PEILE – fullname: HUANG PINTONG – fullname: CHEN JIFAN – fullname: LIN TAO |
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| DocumentTitleAlternate | 超声空化促进活体阳离子脂质-核酸复合体转染的载体及其制备方法和应用 |
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| Notes | Application Number: CN202410102857 |
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| Snippet | The invention discloses a carrier for promoting in-vivo cationic lipid-nucleic acid complex transfection through ultrasonic cavitation and a preparation method... |
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| Title | Carrier for promoting transfection of in-vivo cationic lipid-nucleic acid complex through ultrasonic cavitation as well as preparation method and application of carrier |
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