Brown algae refining method

• Main Authors: PAN MINGXUAN, GENG LIHUA, WANG JING, ZHANG QUANBIN, YUE YANG, WANG HUIMEI, YAN CHANGHUI, HU YADONG, WU NING
• Format: Patent
• Language: Chinese; English
• Published: 05.04.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a brown algae refining method which comprises the following steps: crushing brown algae, transferring the crushed brown algae to countercurrent extraction equipment, and carrying out countercurrent digestion by using a Na2CO3 solution; cooling the digested mixture to room temperature, adjusting the pH value to 6-7, adding immobilized compound enzyme, and hydrolyzing for 10-20 hours in a stirring state; cooling the solution after enzymolysis to room temperature, centrifuging, removing the precipitate, and collecting the supernate; and cooling the supernate to room temperature, desalting by adopting a nanofiltration membrane, performing ultrafiltration on the desalted concentrated solution by using a membrane, respectively collecting permeate and trapped fluid, concentrating, and drying to respectively obtain alginate oligosaccharide and fucoidan polysaccharide sulfate. According to the invention, the two active components, namely alginate oligosaccharide and fucoidan polysaccharide sulf