High-throughput screening method of recombinant engineering protein and proteasome inhibitor

• Main Authors: NI BIN, ZENG QINGJUAN, WANG LI, HUANG XINGCHUN, LIU WENLIANG, HU QIKANG, YU FENGLEI, ZENG JIN
• Format: Patent
• Language: Chinese; English
• Published: 26.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention belongs to the field of biomedical engineering, and provides a novel recombinant protein which comprises a fusion protein formed by a TMEM8B-a protein and a reporter protein, and a novel proteasome inhibitor can be screened with high throughput by using the recombinant protein. The invention also correspondingly provides a high-throughput screening method of the proteasome inhibitor. The screening method is expected to quickly and effectively screen a new generation of proteasome inhibitor medicine which is better in effect, stronger in specificity, smaller in side effect and also plays a role in solid tumors. And for the high-throughput screening method, the whole process can be completed in a living cell state, and cell lysis or addition of an additional color development or luminescence reaction reagent is not needed, so that the high-throughput screening method is simple, convenient, rapid and accurate. The screening system constructed by the invention can be used for very conveniently scree