Construction of multi-target-point-based hypoglycemic evaluation system and application of multi-target-point-based hypoglycemic evaluation system in formula optimization

• Main Authors: XU JIAN, CUI LINGMIN, TANG WAN, LAI FULI, LI WEIQIN, WANG SUQIN, WANG ZHE
• Format: Patent
• Language: Chinese; English
• Published: 08.03.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses construction based on a multi-target hypoglycemic evaluation system and application of the multi-target hypoglycemic evaluation system in formula optimization, which is characterized in that the multi-target hypoglycemic evaluation system is established for the first time by using respectively constructed high-throughput screening models of alpha-amylase, alpha-glucosidase, dipeptidyl kinin IV and protein tyrosine phosphatase 1B inhibitors; the method is more comprehensive, efficient and convenient in screening of active raw materials for reducing blood sugar and optimization of a formula. The composition of the formula is disassembled and optimized from activity comparison of different action targets according to formula optimization, and the hypoglycemic activity and application universality of the formula under multiple targets can be effectively improved. 本发明公开了一种基于多靶点降血糖评价体系构建及其在组方优化中的应用，其首次将分别构建的α-淀粉酶、α-葡萄糖苷酶、二肽激肽酶Ⅳ、蛋白酪氨酸磷酸酶1B抑制剂高通量筛选模型建立多靶点降血糖评价体系，在降血糖活性原料筛选和组方优化中更加全面、高效、便捷。针对组方