Lysosome targeting fluorescent probe as well as preparation and application thereof

• Main Authors: GAO WANXIA, SHU XIJI, LIU WEI, ZHANG YIBIN, SHANG JINTING, WANG HAIPING
• Format: Patent
• Language: Chinese; English
• Published: 05.03.2024
• Subjects: ADHESIVES; CHEMISTRY; DYES; HETEROCYCLIC COMPOUNDS; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; NATURAL RESINS; ORGANIC CHEMISTRY; PAINTS; PHYSICS; POLISHES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; TESTING

The invention discloses a lysosome targeting fluorescent probe and preparation and application thereof, the structural formula of the lysosome targeting fluorescent probe is as shown in formula (I), and a # imgabs0 # preparation method comprises the following steps: S1, in an inert atmosphere, m-phenylenediamine and 2, 2, 2-trifluoroacetophenone are used as raw materials, a heating reflux reaction is performed to obtain a crude product, and the crude product is purified to obtain an intermediate product; and S2.0, adding sodium nitrite into the hydrochloric acid solution of the intermediate product at 0-4 DEG C, carrying out a stirring reaction to obtain a mixture, adding stannous chloride, and carrying out a stirring reaction to obtain the lysosome targeting fluorescent probe. The lysosome targeted fluorescent probe has special affinity for the lysosome, and the hydrazine group of the lysosome targeted fluorescent probe and formaldehyde generate Schiff base, so that the PET effect is reduced, the fluorescenc