Detection kit based on hybridization chain reaction (HCR) fish pathogenic microorganism aeromonas hydrophila metG gene and application thereof
The invention relates to development and application of a detection kit based on a hybridization chain reaction (HCR) fish pathogenic microorganism aeromonas hydrophila metG gene. The detection kit comprises a detected aeromonas hydrophila specific DNA sequence metyl-tRNA synthase, a pair of hairpin...
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Language | Chinese English |
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27.02.2024
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Abstract | The invention relates to development and application of a detection kit based on a hybridization chain reaction (HCR) fish pathogenic microorganism aeromonas hydrophila metG gene. The detection kit comprises a detected aeromonas hydrophila specific DNA sequence metyl-tRNA synthase, a pair of hairpin primers metG-H1 and metG-H2, a fluorophore, a quenching group, a DNA storage solution (1X) and a CaCl2 solution. When in use, the H1 and H2 reagents are added into a water body sample or a bacterial liquid prepared by separating visceral organs and tissues of sick fishes; standing on ice, and carrying out heat shock at a constant temperature of 42 DEG C; quickly placing on ice for cooling after heat shock is completed; and adding a DNA storage solution, placing at room temperature, and detecting by using a fluorescence microscope. And judging whether the sample to be detected contains aeromonas hydrophila or not and the proportion of pathogenic bacteria by observing the luminescence condition of the sample under a |
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AbstractList | The invention relates to development and application of a detection kit based on a hybridization chain reaction (HCR) fish pathogenic microorganism aeromonas hydrophila metG gene. The detection kit comprises a detected aeromonas hydrophila specific DNA sequence metyl-tRNA synthase, a pair of hairpin primers metG-H1 and metG-H2, a fluorophore, a quenching group, a DNA storage solution (1X) and a CaCl2 solution. When in use, the H1 and H2 reagents are added into a water body sample or a bacterial liquid prepared by separating visceral organs and tissues of sick fishes; standing on ice, and carrying out heat shock at a constant temperature of 42 DEG C; quickly placing on ice for cooling after heat shock is completed; and adding a DNA storage solution, placing at room temperature, and detecting by using a fluorescence microscope. And judging whether the sample to be detected contains aeromonas hydrophila or not and the proportion of pathogenic bacteria by observing the luminescence condition of the sample under a |
Author | CUI XIAOJUAN CHEN ZHILAN SUN YUANDONG DENG JIMIN ZHONG LIZHEN ZHANG YIFEI |
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DocumentTitleAlternate | 基于杂交链式反应(HCR)鱼类病原微生物嗜水气单胞菌metG基因的检测试剂盒及其应用 |
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Snippet | The invention relates to development and application of a detection kit based on a hybridization chain reaction (HCR) fish pathogenic microorganism aeromonas... |
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SubjectTerms | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
Title | Detection kit based on hybridization chain reaction (HCR) fish pathogenic microorganism aeromonas hydrophila metG gene and application thereof |
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