Blocking agent for resisting rheumatoid factors as well as preparation method and application of blocking agent

• Main Authors: YANG XIAOLI, LI GUOQIANG, GAO YIZHENG, LIU SHICHENG
• Format: Patent
• Language: Chinese; English
• Published: 01.12.2023
• Subjects: CHEMISTRY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; TESTING

The invention discloses an anti-rheumatoid factor blocking agent as well as a preparation method and application thereof. The preparation method comprises the following steps: performing mouse immunization by using human IgM protein, and preparing a cell strain and a monoclonal antibody; according to the step (1), B/C mouse immunization of human IgG protein and screening for preparing an anti-human IgM specific antibody as a blocking agent from a monoclonal antibody are carried out. The variable region sequence of the RF blocker is provided mainly for a monoclonal antibody which is excellent in RF blocking effect, stable in batch, good in consistency, low in price and wide in application of an immunodetection platform, in-vitro recombination is met, in-vitro affinity maturation operation is facilitated, and stable batch production and forward effect improvement of the RF blocker are achieved. 本发明公开了抗类风湿因子的阻断剂及制备方法，应用，包括使用人IgM蛋白进行小鼠免疫，细胞株以及单克隆抗体制备；根据步骤一)进行人IgG蛋白的B/C小鼠免疫和单克隆抗体制备抗人IgM特异性抗体作为阻断剂使用的筛选。本发明主要针对免疫检测平