Preparation method of Retatrutide

• Main Authors: LI XUEHAO, JI DONGLIANG, GONG YULU, SHI HAIFANG
• Format: Patent
• Language: Chinese; English
• Published: 10.11.2023
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

The invention discloses a preparation method of Retatrutide, and belongs to the technical field of polypeptide synthesis. The synthesis method comprises the following steps: preparation of peptide resin: coupling protected amino acid or protected polypeptide fragments to solid-phase carrier resin one by one according to the sequence of Retatrutide to obtain the peptide resin; preparation of crude peptide: splitting the peptide resin by using a splitting solution to obtain the crude peptide; and preparation of a refined product of the Retatrutide: purifying the crude peptide, so as to obtain the Retatrutide. According to the present invention, the protected polypeptide fragment is adopted to synthesize the Retatrutide, such that the Retatrutide synthesis method has advantages of short time period, low preparation difficulty, less waste liquid discharge, high total yield, low cost, and large-scale production benefiting. 本发明公开了一种Retatrutide的制备方法，属于多肽合成技术领域；该合成方法包括，肽树脂的制备：按Retatrutide的序列顺序，逐个偶联带保护氨基酸或带保护多肽片段至固相载体