Alkaline protease mutant with improved stability and application thereof

• Main Authors: LI YU, LIU FUFENG, ZHANG HUITU, LIU YIHAN, WANG HONGBIN, LU FUPING, GUO ENPING
• Format: Patent
• Language: Chinese; English
• Published: 27.10.2023
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; BEER; BIOCHEMISTRY; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DETERGENT COMPOSITIONS; DETERGENTS; ENZYMOLOGY; FATTY ACIDS THEREFROM; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECOVERY OF GLYCEROL; RESIN SOAPS; SOAP OR SOAP-MAKING; SPIRITS; USE OF SINGLE SUBSTANCES AS DETERGENTS; VINEGAR; WINE

The invention belongs to the technical field of bioengineering, and particularly relates to alkaline protease mutants which are obtained by random mutation through an error-prone PCR technology and have improved stability in two negative surfactants and application of the alkaline protease mutants....