Lactic acid bacteria for expressing ribosome inactivation protein and application of lactic acid bacteria in rotavirus resistance

• Main Authors: SHI CHUNWEI, YANG GUILIAN, YE LIPING, NIU TIANMING, FENG XIZE, YANG JINLONG, FAN SHUHUI, WANG CHUNFENG, CAO XIN, YANG WENTAO
• Format: Patent
• Language: Chinese; English
• Published: 13.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides lactic acid bacteria for expressing ribosome inactivated protein and application of the lactic acid bacteria in rotavirus resistance. A ribosome inactivation protein gene is integrated in the lactic acid bacteria, and the ribosome inactivation protein gene is a ribosome inactivation protein gene PAP or a ribosome inactivation protein gene B17. The lactobacillus can efficiently express PAP and B17 antigen proteins, has good immunogenicity, can induce mucosal immunity, cellular immunity and humoral immunity responses, can be used for preventing and treating rotavirus infection, and has good practical application value. 本申请提供一种表达核糖体失活蛋白的乳酸菌及其在抗轮状病毒中的应用。所述乳酸菌内整合有核糖体失活蛋白基因，其中，所述核糖体失活蛋白基因为核糖体失活蛋白基因PAP或核糖体失活蛋白基因B17。本发明所述乳酸菌能高效表达PAP和B17抗原蛋白，具有良好的免疫原性，能够诱导黏膜免疫、细胞免疫和体液免疫应答，可用于防治轮状病毒感染，具有良好的实际应用价值。