Pancreatic cancer organoid culture solution, culture reagent combination and culture method

• Main Authors: HU HAO, LIU JIANCHUANG, CHENG XI, XIAO RONGRONG, ZHOU YU
• Format: Patent
• Language: Chinese; English
• Published: 29.09.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of biological tissue engineering, and discloses a pancreatic cancer organoid culture solution, which comprises a primary culture solution and a subculture solution, and each of the primary culture solution and the subculture solution comprises a conditioned culture medium, a basic culture medium, a compound antibiotic and a growth factor. According to the characteristics of pancreatic cancer organs, an auxiliary protein conditioned medium and FGF are subtracted from a primary culture solution, and the concentration of penicillin-streptomycin is increased, so that the pollution risk of primary tissues can be inhibited in primary culture, and pancreatic cancer tumor cells can be purified; in addition, the optimized subculture solution can maintain multi-generation subculture and rapid propagation of pancreatic cancer, and the proliferation speed is ensured. The culture medium is suitable for culturing human pancreatic cancer organs. The invention further discloses a