Expression vector, strain, method and application for biosynthesizing nicotinamide ribose
The invention relates to the technical field of nicotinamide ribose, in particular to an expression vector, a strain, a method and application for biosynthesis of nicotinamide ribose. The expression vector carries a first coding gene of an export protein MdtL and a second coding gene of ribose phosp...
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Main Authors | , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
01.08.2023
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Subjects | |
Online Access | Get full text |
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Summary: | The invention relates to the technical field of nicotinamide ribose, in particular to an expression vector, a strain, a method and application for biosynthesis of nicotinamide ribose. The expression vector carries a first coding gene of an export protein MdtL and a second coding gene of ribose phosphate transferase NAMPT, the nucleotide sequence of the first coding gene is as shown in SEQ ID NO.1, the nucleotide sequence of the second coding gene is as shown in SEQ ID NO.2, the expression vector secretes the ribose phosphate transferase in an extracellular mode after being expressed by host bacteria of the expression vector, and the ribose phosphate transferase is expressed by the host bacteria of the expression vector. The synthesis of nicotinamide into nicotinamide ribose is catalyzed. The bacillus into which the expression vector is transferred can express ribose phosphate transferase extracellularly, and the content of nicotinamide ribose produced by fermentation of the bacillus is far higher than the bio |
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Bibliography: | Application Number: CN202310336492 |