Nucleic acid aptamers for specifically recognizing soybean trypsin inhibiting factor, kit for detecting inhibiting factor and application of nucleic acid aptamers

• Main Authors: HAN CHENGGANG, ZHU DAN, ZHAO LONGGANG, ZHAO PING, HAN LEI, FEI LIN, SUN SHIMIN
• Format: Patent
• Language: Chinese; English
• Published: 01.08.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses a pair of nucleic acid aptamers for specifically recognizing a soybean trypsin inhibiting factor, a kit for detecting the inhibiting factor and application of the nucleic acid aptamers. The nucleic acid aptamers for specifically recognizing the soybean trypsin inhibiting factor comprise a nucleic acid aptamer 1 of which the nucleotide sequence is SEQ ID NO. 1 and a nucleic acid aptamer 2 of which the nucleotide sequence is SEQ ID NO. 2. The kit for detecting the soybean trypsin inhibiting factor comprises a nanoparticle combined with the nucleic acid aptamer 1 and a pH indicator molecule, and a vessel modified with the nucleic acid aptamer 2. The nucleic acid aptamers Aptameer1 and Aptameer2 specifically recognize different parts of the soybean trypsin inhibiting factor respectively and are combined with the soybean trypsin inhibiting factor to form a specific sandwich structure with PH indicator molecules, so that the detection of the soybean trypsin inhibiting factor is realized, the