Application of OsCOL5 gene in regulation and control of heading stage of rice

• Main Authors: ZHAN XIAODENG, CHENG SHIHUA, ZHANG YINGXIN, LIU QUN'EN, WEN XIAOXIA, WU WEIXUN, CAO LIYONG
• Format: Patent
• Language: Chinese; English
• Published: 28.07.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses an application of an OsCOL5 gene in regulation and control of a heading stage of rice. The OsCOL5 gene is from a rice variety Nipponbare, and the gene encodes a B-box/CCT zinc finger protein with 333 amino acids, and belongs to a CONSTANS-like transcription factor gene family. Researches find that the OsCOL5 is positioned in a cell nucleus and has relatively strong transcriptional self-activation activity, and the expression mode of the OsCOL5 shows obvious rhythmicity. Genetic transformation experiments prove that the OsCOL5 overexpression transgenic plant can delay rice heading under long-day and short-day conditions; the knockout of the OsCOL5 has no obvious phenotypic change, which indicates that the gene may have a function redundancy phenomenon. According to expression analysis and interaction experiment speculation, the OsCOL5 is located at the upstream of Ghd7 and Ehd2 in a rice photoperiod flowering pathway, and interacts with the OsELF3-1 and the OsELF3-2. 本发明公开了OsCOL5基因在调控水稻