EB (Epstein-Barr) virus detection primer probe composition, kit and detection method

• Main Authors: HE XIAOYONG, TAO YONG, LU CHENGHUI
• Format: Patent
• Language: Chinese; English
• Published: 04.07.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides an EB (Epstein-Barr) virus detection primer probe composition, a kit and a detection method, and relates to the technical field of molecular diagnosis, the EB virus detection primer probe composition comprises at least one EB virus primer probe group; each EB virus primer probe group comprises a pair of amplification primers; the first probe is complementary with a positive-sense strand of a target fragment; the second probe is complementary with an antisense strand of the target fragment; each probe is modified with two luminous groups which are respectively positioned at the 5'end and the middle position of the probe, and the EB virus detection primer probe composition relieves the problem that in the prior art, the EB virus detection sensitivity of a fluorescent PCR method is not low enough. 本发明提供了一种EB病毒检测引物探针组合物、试剂盒和检测方法，涉及分子诊断技术领域，包括至少一组EB病毒引物探针组；每个EB病毒引物探针组包括一对扩增引物；以及与目的片段正义链互补的第一探针和与目的片段反义链互补的第二探针；每个探针修饰有两个发光基团，分别位于探针的5'端和中间位置，EB病毒检测引物探针组合物缓解了现有技术中存在的荧光PCR法检测EB病毒灵敏度不够低的问题。