Method for maintaining and amplifying human primordial germ cells/human primordial germ cell-like cells

Provided are: a method for maintaining and amplifying human primordial germ cells (hPGC) or human primordial germ cell-like cells (hPGCLC) derived from human pluripotent stem cells; the method includes culturing hPGCLC in the presence of (i) forskolin or a phosphodiesterase 4 (PDE4) inhibitor, and (...

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Main Authors SAITO MICHINORI, MURASE YUSUKE
Format Patent
LanguageChinese
English
Published 23.06.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Abstract Provided are: a method for maintaining and amplifying human primordial germ cells (hPGC) or human primordial germ cell-like cells (hPGCLC) derived from human pluripotent stem cells; the method includes culturing hPGCLC in the presence of (i) forskolin or a phosphodiesterase 4 (PDE4) inhibitor, and (ii) one or more cytokines selected from the group consisting of basic fibroblast proliferation factor (bFGF), leukemia inhibitory factor (LIF), and epithelial growth factor (EGF). 本发明提供：人原始生殖细胞(hPGC)或来自人多能干细胞的人原始生殖细胞样细胞(hPGCLC)的维持扩增方法，该方法包括：在(i)佛司可林或磷酸二酯酶4(PDE4)抑制剂、以及(ii)选自碱性成纤维细胞增殖因子(bFGF)、白血病抑制因子(LIF)和上皮生长因子(EGF)的1种以上的细胞因子的存在下培养hPGCLC。
AbstractList Provided are: a method for maintaining and amplifying human primordial germ cells (hPGC) or human primordial germ cell-like cells (hPGCLC) derived from human pluripotent stem cells; the method includes culturing hPGCLC in the presence of (i) forskolin or a phosphodiesterase 4 (PDE4) inhibitor, and (ii) one or more cytokines selected from the group consisting of basic fibroblast proliferation factor (bFGF), leukemia inhibitory factor (LIF), and epithelial growth factor (EGF). 本发明提供：人原始生殖细胞(hPGC)或来自人多能干细胞的人原始生殖细胞样细胞(hPGCLC)的维持扩增方法，该方法包括：在(i)佛司可林或磷酸二酯酶4(PDE4)抑制剂、以及(ii)选自碱性成纤维细胞增殖因子(bFGF)、白血病抑制因子(LIF)和上皮生长因子(EGF)的1种以上的细胞因子的存在下培养hPGCLC。
Author MURASE YUSUKE
SAITO MICHINORI
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DocumentTitleAlternate 人原始生殖细胞/人原始生殖细胞样细胞的维持扩增方法
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SubjectTerms BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Title Method for maintaining and amplifying human primordial germ cells/human primordial germ cell-like cells
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