Molecular marker based on chloroplast genome microsatellite and method for identifying walnut germplasm and genetic relationship thereof

• Main Authors: SHU XIUGE, SUN CHAO, WANG JING, WANG CUIXIANG, LIANG JING, LIANG YAN, HAN CHUANMING, ZHONG WEIGUO, PEI DONG, SONG XIAOBO, MA ANBAO
• Format: Patent
• Language: Chinese; English
• Published: 18.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a chloroplast genome microsatellite-based molecular marker and a method for identifying walnut germplasm and genetic relationship thereof, and belongs to the technical field of SSR molecular markers. According to the invention, SSR molecular markers with walnut intra-genus variety identification value are obtained based on walnut chloroplast group gene screening and comprise SSR-P8 and SSR-P9, and primers with good polymorphism and identification effect are synthesized. The method can be used for identifying the germplasm, the interspecific hybrid and the genetic relationship of the five species, namely the Juglans nigra, the Juglans major, the Juglans hinsii, the Juglans microcapa and the Juglans regia, and can be used for identifying the germplasm, the interspecific hybrid and the genetic relationship of the five species, namely the Juglans nigra, the Juglans major, the Juglans hinsii, the Juglans microcapa and the Juglans regia. 本发明涉及一种基于叶绿体基因组微卫星的分子标记及鉴定核桃种质及其亲缘关系的方法，属于SSR分子标记技术领域