Application of high-temperature sensitive type rice leaf whitening gene OsHCD1 in regulation and control of rice leaf color traits

• Main Authors: PAN GANG, ZHANG JIE, ZHAO JIAYI, XIANG LONGYI, CHEN JIAMENG, JIN ZEYAN, CHENG FANGMIN, CHEN YANYAN
• Format: Patent
• Language: Chinese; English
• Published: 07.04.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses an application of a high-temperature sensitive type rice leaf whitening gene OsHCD1 in regulation and control of rice leaf color traits. The genome sequence of the whitening gene OsHCD1 is as shown in SEQ ID NO. 1. According to the invention, a high-temperature sensitive whitening gene OsHCD1 is cloned from a rice leaf whitening mutant oshcd1 through a map-based cloning technology; knocking out the OsHCD1 gene in the rice two-line sterile line by using a gene editing system to obtain a two-line sterile line carrying a leaf whitening character; the gene OsHCD1 can be applied to the field of rice breeding, and has important significance on false hybrid removal and sterile line seed purity identification. 本发明公开了一种高温敏感型水稻叶片白化基因OsHCD1在调控水稻叶色性状中的应用，该白化基因OsHCD1的基因组序列如SEQ ID NO.1所示。本发明通过图位克隆技术从水稻叶片白化突变体oshcd1中克隆获得高温敏感型白化基因OsHCD1；并利用基因编辑系统敲除水稻两系不育系中的OsHCD1基因，获得携带叶片白化性状的两系不育系；该基因OsHCD1可应用于水稻育种领域，对假杂种去除及不育系种子纯度鉴定具有重要意义。