Ox-bile-resistant bezoar transforming bacterium and application thereof

• Main Authors: ZHAO JINLONG, XU LINGCHUAN, WANG KANGCHENG, DOU YANLING, WANG HOUWEI, SUN YAN
• Format: Patent
• Language: Chinese; English
• Published: 28.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the field of resource development and utilization of oxgall-resistant bezoar transforming bacteria, and particularly relates to an oxgall-resistant bezoar transforming bacterium and application thereof. The characteristic base sequence of 16S rDNA of the oxgall-resistant bezoar transformed bacterium is shown as SEQ No.1, characteristic base sites comprise 265-A, 275-C, 280-G, 282-A, 537-A and 581-A, the oxgall-resistant bezoar transformed bacterium is unique oxgall citrobacter, and the preservation number of the strain is CGMCC No.24893. The oxgall-resistant bezoar transformed bacterium is applied to fermentation production of in-vitro cultured bezoar, and the content of bilirubin and cholic acid fermented at 30 DEG C for 16 days is increased by more than two times compared with that of natural oxgall. The strain NDZNM-01 disclosed by the invention can tolerate oxgall with a broad-spectrum bacteriostatic effect, the survival time in pure oxgall is longer than 9 days, the survival time