Application of gene editing knockout TaSK2A in increasing wheat grain length

• Main Authors: LYU JINYANG, HAN CHAO, BAI MINGYI, LEE KEUN-YOUNG, FAN MIN
• Format: Patent
• Language: Chinese; English
• Published: 10.03.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses an application of gene editing knockout TaSK2A in increasing wheat grain length, which comprises the following steps: designing a single-chain guide RNA (Ribonucleic Acid) of a targeted TaSK2A gene, and constructing a plant binary expression vector pBUE411-TaSK2A for knocking out the TaSK2A gene in a wheat body; the plant binary expression vector is transformed into common wheat through agrobacterium to obtain transgenic wheat with the TaSK2A gene knocked out, and the grain length of wheat seeds is increased. Experiments prove that the transgenic wheat with the TaSK2A gene knocked out realizes the increase of the grain length and the thousand grain weight of the wheat seeds. The application of the invention provides a feasible method for realizing rapid wheat breeding and increasing wheat yield by utilizing a genetic engineering technology, and has important breeding application value and wide market application prospect. 本发明公开了一种利用基因编辑敲除TaSK2A在增加小麦籽粒长度中的应用，是通过设计靶向TaSK2A基因的单链引导RNA，构建敲除