Method for generating human parenchymal hepatic cells by inducing IPS cell differentiation in vitro

• Main Author: WANG XINJUAN
• Format: Patent
• Language: Chinese; English
• Published: 09.12.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for generating human parenchymal hepatic cells by inducing IPS cell differentiation in vitro. The method specifically comprises the following steps: (1) culturing human embryonic stem cells on a differentiation culture medium I; (2) culturing the cells obtained in the step (1) on a differential medium II, and differentiating to form directional endoderm cells; (3) culturing the cells obtained in the step (2) on a differential medium III; (4) culturing the cells obtained in the step (3) on a differential medium IV, and differentiating to form hepatic progenitor cells; (5) culturing the cells obtained in the step (4) on a differential medium V, and differentiating to obtain liver-like tissues; the invention specifically discloses the specific composition of each differential culture medium. The invention relates to the technical field of directional differentiation culture of stem cells, and particularly provides a method for generating human parenchymal hepatic cells by inducin