Construction method of recombinant virus for expressing reporter protein by taking Getavirus as vector

• Main Authors: WEI ZUZHANG, REN TONGWEI, MO QINGRONG, MIN XIANGLING
• Format: Patent
• Language: Chinese; English
• Published: 06.12.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an expression vector of a Getavirus serving as an exogenous gene. Accordingly, a corresponding construction method is also established based on a GETV reverse genetic manipulation platform. The method comprises the following steps: designing a corresponding series of primers according to a genome sequence of the Geitavirus and a gene sequence of an exogenous reporter protein, constructing recombinant plasmids for expressing the reporter protein between a virus Cap protein and an E3 protein and at a 3'end of the E1 protein by utilizing a genetic engineering method, and rescuing a recombinant virus for expressing the reporter protein; or different report protein genes are respectively inserted between the Cap protein and the E3 protein of the virus and at the 3'end of the E1 protein at the same time, recombinant plasmids capable of expressing the two different report proteins at the same time are constructed, and the recombinant virus capable of expressing the two different report protei