Application of enzyme-linked immunodot assay in dapsone syndrome diagnosis

• Main Authors: SUN LELE, LIU HONG, ZHANG FUREN
• Format: Patent
• Language: Chinese; English
• Published: 28.10.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses an application of an enzyme-linked immune spot experiment in dapsone syndrome diagnosis. The invention provides application of a substance for detecting whether a peripheral blood mononuclear cell is positive in an IFN-gamma and/or IL-5 release Elispot experiment under the stimulation of dapsone in preparation of a product for diagnosis or auxiliary diagnosis of whether a subject is a dapsone syndrome patient. The substance comprises the following components: 1) dapsone as a stimulating drug; 2) a reagent and/or equipment for acquiring peripheral blood mononuclear cells of a subject; and 3) a reagent and/or equipment used for detecting whether an IFN-gamma and/or IL-5 release Elispot experiment is positive. According to the invention, an Elispot experiment combined with IFN-gamma/IL-5 release is successfully established, and an effective technical means is provided for diagnosis of dapsone syndrome. 本发明公开了酶联免疫斑点实验在氨苯砜综合征诊断中的应用。本发明提供了用于检测外周血单个核细胞在氨苯砜刺激下IFN-γ和/或IL-5释放Elispot实验是否为阳性的物质在制备