ELISA (enzyme-linked immunosorbent assay) kit for detecting specific IgA (immunoglobulin A) antibody of food protein and application thereof

• Main Authors: ZHU YUN, WANG FENGYING, CHEN RUYUE, LI XIAOZHONG, MIAO MEIHUA, HU XIAOHAN, LIN QIANG
• Format: Patent
• Language: Chinese; English
• Published: 14.10.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a food protein specific IgA antibody detection ELISA kit and application thereof. The food protein specific IgA antibody detection ELISA kit comprises a diluent, an ELISA plate coated with an antigen, a plate washing solution, a standard substance, a negative reference substance, a biotin secondary antibody, avidin-HRP, a developing solution and a stop solution. The diluent is prepared from PVP-K30, sodium chloride, potassium chloride, monopotassium phosphate, dipotassium phosphate, lauryl sodium sulfate, sodium azide, dithiothreitol, polyethylene glycol 2000, mannitol, trehalose and water. The diluent of the kit enables food protein to be more stable in the coating process, the coating effect is improved, and the influence of the difference of the antigen coating process on the detection result is reduced; the avidin-biotin ELISA kit for detecting the specific IgA antibody of the food protein is successfully established by taking the total IgA antibody as a standard substance. 本发明提供了一种