Hybridoma cell strain secreting porcine IFN (interferon)-lambda4 monoclonal antibody, monoclonal antibody secreted by hybridoma cell strain and application of monoclonal antibody

• Main Authors: DUAN PANPAN, LU YOUXIN, FANGPU COUNTY, PENG XUAN, LIU LIZHI, XIAO XUN, LIU SHIYU, FANG LIURONG, ZHOU YANRONG, XIAO SHAOBO
• Format: Patent
• Language: Chinese; English
• Published: 30.09.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention belongs to the field of immunology, and relates to a hybridoma cell strain for secreting a porcine IFN (interferon)-lambda4 monoclonal antibody, the monoclonal antibody secreted by the hybridoma cell strain and application of the monoclonal antibody. The preservation number of the hybridoma cell strain lambda 4-1A2 is CCTCC (China Center For Type Culture Collection) NO: C2022132. A light chain variable region of the monoclonal antibody comprises a CDR1 as shown in SEQ ID NO: 3, a CDR2 with an amino acid sequence of LVS and a CDR3 as shown in SEQ ID NO: 4; the heavy chain variable region comprises a CDR1 as shown in SEQ ID NO: 7, a CDR2 as shown in SEQ ID NO: 8 and a CDR3 as shown in SEQ ID NO: 9. The monoclonal antibody can be used for detecting the sIFN-lambda 4. The monoclonal antibody secreted by the hybridoma cell strain lambda4-1A2 disclosed by the invention has good specificity, and can specifically recognize sIFN-lambda4 recombinant protein expressed by pronucleus and eukaryotic expressio