Human liver chimeric non-human animals with defective p450 oxidoreductase and methods of use thereof

• Main Authors: DIEGUEZ MARIA DE LAS MERCEDES BARBA, BISSIG KLAUS-DIETER, PANKOVICH FRANCIS PATRICK
• Format: Patent
• Language: Chinese; English
• Published: 09.08.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The present invention relates to human liver chimeric non-human animals with defective P450 oxidoreductase and methods of use thereof. The present disclosure provides chimeric non-human animals comprising human hepatocytes, methods for making chimeric non-human animals comprising human hepatocytes, and screening and identifying any type of drug (generally small molecule drug) that may affect human liver function and any other body function using chimeric non-human animals comprising human hepatocytes The invention relates to a method for preparing metabolites. 本发明涉及具有缺陷性P450氧化还原酶的人肝嵌合非人动物及其使用方法。本公开内容提供包含人肝细胞的嵌合非人动物、用于制备包含人肝细胞的嵌合非人动物的方法和利用包含人肝细胞的嵌合非人动物筛选和鉴定可能影响人肝功能和任何其他身体功能的任何类型药物(一般地为小分子药物)的代谢物的方法。