Development and application of KASP marker of tomato ps-2 gene

• Main Authors: ZHANG YANDI, JIA MINGCHAO, JIA ZHIQI, LI JISUO, KANG DONGMU, LI YING
• Format: Patent
• Language: Chinese; English
• Published: 05.07.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses development and application of a KASP marker of a tomato ps-2 gene. The development of the KASP marker of the tomato ps-2 gene is realized through tomato material selection, tomato leaf DNA extraction and PCR amplification by using a KASP primer. Amplification products are detected and analyzed; the KASP marker of the tomato ps-2 gene can be used for molecular marker-assisted breeding of the tomato ps-2 male sterility gene; the method can be used for accurately identifying male sterile materials and male fertile materials, and has high practicability in screening of tomato male sterile plants. 本发明公开了番茄ps-2基因的KASP标记开发及应用，通过番茄材料选定，番茄叶片DNA提取，使用KASP引物进行PCR扩增实现番茄ps-2基因的KASP标记开发；并对扩增产物的检测和分析，本发明番茄ps-2基因的KASP标记可用于番茄ps-2雄性不育基因的分子标记辅助育种；对雄性不育材料和雄性可育材料均能够准确地进行鉴定，在番茄雄性不育植株的筛选中具有较高的实用性。