Double-probe composition for detecting colorectal cancer and application thereof

• Main Authors: ZHANG HONGYAN, MA FENGJIE, BAEK YONG-WOON, BAO WENZHI, JIN RIHU, GU HENGSEN
• Format: Patent
• Language: Chinese; English
• Published: 28.06.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a double-probe composition for detecting colorectal cancer and application of the double-probe composition. The double-probe composition comprises a target gene SDC2 and a reference gene ACTB, the target gene SDC2 comprises S-F, S-R, S-P1 and S-P2, and the nucleotide sequence of the target gene SDC2 is shown as SEQ ID NO.1-4; the reference gene ACTB comprises A-F, A-R and A-P1, and the nucleotide sequences of the reference gene ACTB are as shown in SEQ ID NO.5-7. The double-probe composition for detecting the colorectal cancer can be applied to preparation of a kit for detecting the colorectal cancer. By adopting the double-probe fluorescent PCR technology, the sensitivity of the detection reagent can be obviously improved, and the detection reagent has the advantages of high sensitivity and strong specificity. 本发明公开了一种用于检测结直肠癌的双探针组合物及其应用，包括靶标基因SDC2和内参基因ACTB，靶标基因SDC2包括S-F、S-R、S-P1和S-P2，其核苷酸序列如SEQ ID NO.1~4所示；内参基因ACTB包括A-F、A-R和A-P1，其核苷酸序列如SEQ ID NO.5~7所示。本发明中的用于检测结直肠癌的双探针组合物可应用在制备检测结直肠癌