具有自组装特性和生物矿化功能的藤壶胶蛋白20K衍生多肽、其制备方法和应用

• Format: Patent
• Language: Chinese
• Published: 07.11.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

本发明涉及具有自组装特性和生物矿化功能的藤壶胶蛋白20K衍生多肽、其制备方法和应用，通过同源序列比对获得了潜在功能片段cp20k-p3(p:peptide)。研究表明，该多肽具备独特的自组装特性，并能够显著地促进小鼠骨髓间充质干细胞的增殖及成骨分化。通过丙氨酸扫描(Alanine Scanning)逐个替换cp20k-p3中的半胱氨酸，获得了4个多肽突变体，比较发现二硫键的形成对于多肽的自组装形貌及生物矿化功能具有显著的影响。不仅为破解cp20k的生物功能提供了线索，同时也为骨组织工程提供了一种全新的功能材料。 The invention relates to a barnacle glue protein 20K derived polypeptide with a self-assembly characteristic and a biomineralization function as well as a preparation method and application of the barnacle glue protein 20K derived polypeptide. A potential functional fragment cp20k-p3 (p: peptide) is obtained through homologous sequence alignment. Researches show that the polypeptide has a unique self-assembly characteristic, and can significantly promote proliferation and osteogenic differentiation of mouse bone marrow mesenchymal stem cells. Cysteine in cp20k-p3 is replaced one by one through alanine scanning, four polypeptide mutants are obtained, and it is found through comparison that formation of disulfide bonds has remarkable influences on the self-