Method for detecting pyrrolizidine alkaloids in honey

• Main Authors: XU SHULING, LI AIFENG, XUE QINGWANG, JOO SU-HWAN, SONG YAQIAN
• Format: Patent
• Language: Chinese; English
• Published: 07.06.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a method for efficiently and quickly detecting PAs (Pyrrolidine alkaloids) in honey without sample pretreatment, which comprises the following steps of: (1) synthesizing a surface enhanced laser Raman spectroscopy (SERS) substrate of nano silver, (2) detecting the PAs in honey, (3) detecting the PAs in honey, (4) detecting the PAs in honey, (5) detecting the PAs in honey, (6) detecting the PAs in honey, (6) detecting the PAs in honey, and (7) detecting the PAs in honey. (2) characterizing the nano-silver SERS substrate; (3) carrying out qualitative and quantitative analysis on four PAs based on SERS (Surface Enhanced Raman Scattering); and (4) detecting PAs in honey based on SERS (Surface Enhanced Raman Scattering). Compared with the existing PAs detection methods (liquid chromatography, enzyme-linked immunosorbent assay, gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry), the method does not need any sample pretreatment operation, has the advantages of