Detection method for rapidly distinguishing yersinia enterocolitica from yersinia intermediary

• Main Authors: WANG WEI, ZHANG QIUYAN, JIANG YINGHUI, TANG JING, LI ZHENGYI, WU LINA, JIA JUNTAO, YIN PEIJUN, YANG JIELIN
• Format: Patent
• Language: Chinese; English
• Published: 27.05.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a detection method for quickly distinguishing yersinia enterocolitica and yersinia intermediary, which is characterized in that a peptide fingerprint spectrum of the yersinia enterocolitica and the yersinia intermediary is detected by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and the yersinia enterocolitica and the yersinia intermediary can be distinguished according to different characteristic peaks of the two yersinia enterocolitica. And identifying the characteristic protein, and distinguishing the yersinia enterocolitica from the yersinia intermediary. According to the method, the identification time is shortened by 1-2 days compared with that of an existing traditional biochemical experiment, the yersinia enterocolitica and the yersinia intermediary can be rapidly distinguished, and rapid detection is achieved. The method is high in automation degree and short in detection time, the probability that front-line detection personnel make cont