Chip kit capable of simultaneously detecting animal and plant-derived allergen components in food

• Main Authors: FAN ANNI, LI JIAO, YANG CHUNJIA, SHE ZHIYUN, CHEN MANYING, ZHAO LING, LIN YAOWEN, ZHOU CHENQING, ZHU XIAODIAN, ZHANG JUAN, HUANG BAOYING, LIU HAIQING, LIANG YUBIN
• Format: Patent
• Language: Chinese; English
• Published: 10.05.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a chip kit capable of simultaneously detecting animal and plant-derived allergen components of food, which comprises an LAMP (loop-mediated isothermal amplification) primer composition and an LAMP primer group for detecting the animal and plant-derived allergen components of the food, wherein the LAMP primer group comprises nucleotide sequences as shown in SEQ ID NO: 1 to SEQ ID NO: 42; the LAMP primer group for detecting the animal-derived components in the food comprises nucleotide sequences as shown in SEQ ID NO: 43 to SEQ ID NO: 62. According to the chip kit capable of simultaneously detecting animal and plant-derived allergen components in food, the animal and plant-derived allergen components in food can be rapidly, accurately and synchronously detected, the sensitivity and the specificity are improved, and the detection period is shortened. The visual requirement can be met in a mode of judging a detection result through an amplification curve, so that the method has the characte