Application and detection reagent for screening allopurinol tolerant gene methylation marker

• Main Authors: ZHAO MING, LIU YU, LU QIANJIN
• Format: Patent
• Language: Chinese; English
• Published: 10.05.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention relates to application and a detection reagent for screening allopurinol tolerant gene methylation markers. The marker comprises an HLA-B * 58: 01 allele, and methylation level detection of HLA-B gene, TRAF1 gene, ITGB2 gene, BCL2 gene, ITGB2-AS1 gene, REPIN1 gene, JPH3 gene, KCNJ1 gene, METTL11B gene and ACSL-1 gene. The method is mainly used for evaluating the risk that a subject suffers from severe drug rash caused by allopurinol, and the sensitivity and specificity of detecting and screening the severe drug rash caused by allopurinol through pure HLA-B * 58: 01 allele are remarkably improved; particularly, the method has important clinical significance on identification of purinol tolerant population with positive HLA-B * 58: 01 allelic genes and intolerant population with negative HLA-B * 58: 01 allelic genes. The method is simple and convenient to operate, high in detection specificity and sensitivity, short in time consumption, small in required sample amount, easy to clinically populariz