Biotin-streptavidin cleavage compositions and library fragment cleavage

• Main Authors: CHIANO, VINCENT, J, CAO DAN, KHURANA TARUN KUMAR, SHAW STEPHEN M, WANG CLIFFORD LEE, XU HONGXIA, KAPPEL FRANZ, WU YIR-SHYUAN, YOO TAESANG, OKUMS, BRIAN, FISHER JEFFREY S
• Format: Patent
• Language: Chinese; English
• Published: 04.03.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

One example of a biotin-streptavidin cleavage composition includes a carboxamide reagent and a salt buffer. The carboxamide agent is present in the biotin-streptavidin cleavage composition in an amount ranging from about 10% to about 50% based on the total volume of the biotin-streptavidin cleavage composition. The salt buffer solution forms the balance of the biotin-streptavidin protein splitting composition. In some examples, library fragments are cleaved from a solid support using the biotin-streptavidin cleavage compositions. In other examples, library fragments are lysed from a solid support using other mechanisms. 生物素-链霉抗生物素蛋白裂解组合物的一个示例包含甲酰胺试剂和盐缓冲液。基于所述生物素-链霉抗生物素蛋白裂解组合物的总体积，所述甲酰胺试剂以约10％至约50％范围内的量存在于所述生物素-链霉抗生物素蛋白裂解组合物中。所述盐缓冲液构成所述生物素-链霉抗生物素蛋白裂解组合物的余量。在一些示例中，使用所述生物素-链霉抗生物素蛋白裂解组合物从固体支持物裂解文库片段。在其他示例中，使用其他机制从固体支持物裂解文库片段。