METHOD FOR NANOPORE RNA CHARACTERISATION

• Main Authors: JAYASINGHE LAKMAL, WHITE JAMES, HERON ANDREW JOHN, GARALDE DANIEL RYAN, TURNER DANIEL JOHN
• Format: Patent
• Language: Chinese; English
• Published: 28.01.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto. 本发明涉及通过随着目标RNA多核苷酸相对于跨膜孔移动，获取一个或多个测量值来表征目标RNA多核苷酸的新方法。通过DNA解旋酶控制该移动。本发明也涉及修饰的RNA构建体，其中所述RNA多核苷酸已被修饰以增强DNA解旋酶对其的结合。