Bacillus badius mutant strain for high yield of chlorogenic acid

• Main Authors: FAN XIANWEI, LI YOUZHI, LI KE, GONG BINTING
• Format: Patent
• Language: Chinese; English
• Published: 28.01.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a bacillus badius mutant strain for high yield of chlorogenic acid, which is named as bacillus badius UWB19-14 and preserved in China Center for Type Culture Collection on May 17, 2021, the preservation number is CCTCC M 2021547, and the preservation address is No. 299 on Bayi road in Wuchang District, Wuhan City, Hubei Province. According to the bacillus badius UWB19-14, an existing strain is subjected to ultraviolet-microwave mutagenesis to obtain a mutant strain, it is found through fermentation culture that the chlorogenic acid fermentation capacity of the UWB19-14 is remarkably improved, and compared with the chlorogenic acid yield in the prior art, the bacillus badius UWB19-14 belongs to a high-chlorogenic-acid-yield strain. 本发明公开了一株高产绿原酸的栗褐芽孢杆菌诱变菌株，命名为栗褐芽孢杆菌(Bacillus badius)UWB19-14，于2021年05月17日保藏于中国典型培养物保藏中心，保藏号为CCTCC M 2021547，保藏地址为湖北省武汉市武昌区八一路299号。本发明栗褐芽孢杆菌UWB19-14是利用现有菌株经过紫外-微波诱变获得的一株突变菌株，通过发酵培养发现UWB19-14发酵绿原酸的能力显著增强，与现有技术的绿原酸产量相比，属于高产绿原酸菌株。