Method for cultivating new Rosa sp. variation variety by using chemical mutagenic agent

• Main Authors: DU HUI, LU PING, LI CHENGXIU, ZHONG FENGWEI, SUN ZHONGKUI, ZHU CUICUI, REN HONGJIAN, ZHANG ANQI, CHENG TIANTIAN, YU YONGCHANG, ZHANG LIN, YAN YU
• Format: Patent
• Language: Chinese; English
• Published: 02.11.2021
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention discloses a method for cultivating a new Rosa sp. variation variety by a using chemical mutagenic agent. The method specifically comprises the following steps of: storing Rosa sp. seeds in sand, germinating, sowing in a hole tray, mixing pingyangmycin and a phosphate buffer solution to prepare a pingyangmycin solution, and mixing the pingyangmycin and an MS liquid culture medium to prepare an MS nutrient solution; when young seedlings come out of the soil, cotyledons are flattened and true leaves germinate, putting the young seedlings into the MS nutrient solution for carrying out hydroponic treatment, treating the growing points of the stem tips of the young seedlings by adopting the pingyangmycin solution, treating once in the morning, at noon and at night every day, continuously treating for 2-4 days, washing the growing points of the stem tips of the young seedlings and the roots of the young seedlings with clear water after the treatment is completed, and transferring the young seedlings in