Temperature regulation and control method for antibody drug production

• Main Authors: TANG PEIFENG, WENG YONGYAN
• Format: Patent
• Language: Chinese; English
• Published: 22.10.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a temperature regulation and control method for antibody drug production. The method comprises the following steps: model data acquisition: respectively culturing antibody drug culture materials at different levels of constant temperatures to obtain culture data; dynamic modeling: constructing a Monod dynamic model related to the culture data, including a dynamic basic equation, and a dynamic equation of a nutrient substrate, a cell metabolite and an antibody product; processing of model parameters: calculating a Monod kinetic model to obtain kinetic parameters, acquiring a relationship among an antibody product concentration, a culture temperature and culture time, and conducting predicting to obtain a temperature control strategy required for obtaining the highest target antibody product concentration; and regulation and control of the culture temperature: regulating and controlling the production temperature of an antibody drug according to a temperature control strategy. By establi