Protease refining and drying method

• Main Authors: LIU HANLING, LIU YAN, LIANG ZHI, ZENG JIWEI, HUANG JU
• Format: Patent
• Language: Chinese; English
• Published: 03.08.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a protease refining and drying method comprising the steps: firstly extracting a crude protease raw material by using a sodium salt solution to improve the solubility of protease, adding a flocculating agent to flocculate and precipitate impurities in the protease solution, and removing the flocculated and precipitated impurities to obtain a relatively pure protease extracting solution; then further filtering and purifying the protease extracting solution by adopting a bag filtration method and a microfiltration method in sequence, then concentrating and desalting by utilizing ultrafiltration to obtain a pure protease concentrated solution; and finally, carrying out vacuum freeze drying on the protease concentrated solution by adopting a vacuum freeze drying machine. The protease refining method is simple and high in efficiency, and the obtained protease is high in purity, high in activity, stable and easy for industrial production. The protease concentrated solution is dried by adopti