Aptamer of sialic acid-binding immunoglobulin-like lectin-15 protein and application thereof

• Main Authors: ZHOU LEIJI, HUANG MENGJIAO, SONG YANLING, WEI XINYU, CHEN FUDE, YANG CHAOYONG, WU QIUYUE
• Format: Patent
• Language: Chinese; English
• Published: 16.07.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses an aptamer of sialic acid-binding immunoglobulin-like lectin-15 (Siglec-15) protein. The aptamer comprises nucleotide sequences as shown in SEQ IDNO: 1- SEQ ID NO: 7. The aptamer provided by the invention can provide rapid and accurate detection on Siglec-15 protein. Meanwhile, The aptamer can block the interaction with T cell surface receptors, and reactivate proliferation of T cells to a certain extent, thereby normalizing immunity in tumor microenvironment without causing serious autoimmune response. The aptamer for Siglec-15 can be used as a medicine for cancers with high Siglec-15 expression in tumor microenvironment, and becomes a potential nucleic acid medicine for tumor immunotherapy. The aptamer of the present invention is possibly effective for patients who have no response to anti-PD-L1 treatment, and further supplements existing tumor diagnosis and immunotherapy systems. 本发明公开了一种唾液酸结合性免疫球蛋白样凝集素-15(Sialic acid-bindingimmunoglobulin-like lectin-15，Siglec-15)蛋白的核酸适配体，所述的核酸适配体包