METHOD FOR PRODUCING INTESTINAL CELLS FROM PLURIPOTENT STEM CELLS

• Main Authors: SHIRAKI NOBUAKI, MAEDA KAZUYA, WATANABE TERUHIKO, KUME SHOEN, ISHIKAWA MASAYA, KUSUHARA HIROYUKI
• Format: Patent
• Language: Chinese; English
• Published: 11.06.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided is a method for producing intestinal cells derived from pluripotent stem cells, the produced intestinal cells exhibiting functions which are similar to those of actual intestinal cells. This method for producing intestinal cells uses a culture for differentiation into intestinal cells, which is characterized by containing a GSK3 inhibitor and one type selected from the group consisting of an activating agent for hepatocyte growth factor receptors, adrenal cortex hormone, calcitriol, and dimethyl sulfoxide. 作为制作显示与实际的肠细胞接近的功能的多能干细胞来源的肠细胞的方法，提供肠细胞的制作方法，其中，使用以包含GSK3抑制剂、和选自肝细胞生长因子受体的激活剂、肾上腺皮质激素、钙三醇、和二甲基亚砜中的至少1种为特征的肠细胞分化用培养基。