Low-temperature exoinulinase mutant MutA122delta5 with reduced thermal stability and application

• Main Authors: ZHANG RUI, HUANG ZUNXI, LI JUNJUN, CEN XIAOLONG, ZHOU JUNPEI, GAO YANXIU, HAN NANYU, XU BO, WU QIAN, TANG XIANGHUA
• Format: Patent
• Language: Chinese; English
• Published: 13.04.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of genetic engineering and protein modification, in particular to a low-temperature exoinulinase mutant MutA122delta5 with reduced thermal stability and an application. The amino acid sequence of the mutant MutA122delta5 is obtained on the basis of removing 122th to 126th amino acids of wild exoinulinase InuAMN8, namely removing 122th to 126th AAPLPs of the InuAMN8. The sequence of the MutA122delta5 is as shown in SEQ ID NO. 1. Compared with the wild enzyme InuAMN8, the mutant enzyme MutA122delta5 has the advantages that the activity at low temperature is improved, the thermal stability is reduced, the low-temperature production is facilitated, and the catalytic reaction of the enzyme is controlled by the temperature change. The low-temperature exoinulinase mutant MutA122delta5 can be applied to the industries of food, wine brewing, washing and the like. 本发明涉及基因工程及蛋白质改造技术领域，具体来说是一种热稳定性降低的低温外切菊粉酶突变体MutA122Δ5及应用，该突变体MutA122Δ5的氨基酸序列是在去除野生外切菊粉酶InuAMN8的第122位至126位氨基酸基础上得