Sensing detection method based on echo wall mode optical microcavity

• Main Authors: WANG XIUHONG, LU JINGSONG
• Format: Patent
• Language: Chinese; English
• Published: 09.04.2021
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a sensing detection method based on an echo wall mode optical microcavity, and belongs to the technical field of detection. The detection method comprises the following points: (1) selection of an echo wall mode microcavity; (2) combination of the microcavity and an aptamer with a secondary structure; (3) selection of a fluorescent substance pair with a fluorescence resonance energy transfer effect; and (4) specific implementation of detection of biological substances to be detected and chemical molecules. The detection range of the detection method relates to numerous biological substances such as nucleic acid, protein, DNA, RNA, exosome, cells and microorganisms and some chemical molecules, but is not limited to the substances. The method is also suitable for intracellular substance sensing detection. 一种基于回音壁模式光学微腔的传感检测方法，属于检测技术领域。这种检测方法包括了以下几点：(1)回音壁模式微腔的选取，(2)微腔与具有二级结构核酸适体的结合，(3)具有荧光共振能量转移效应的荧光物质对的选取，(4)对待测生物物质及化学分子检测的具体实施方法。此探测方法的探测范围涉及到核酸、蛋白质、DNA、RNA、外泌体，细胞及微生物等众多生物物质以及一些化学分子，但不限