DIRECT IMMUNOASSAY MEASUREMENT OF AUTOANTIBODIES

• Main Authors: ARCURI MARIAH, KRUMM CHERYL, MERABET EDDINE, FREEMAN JENNY
• Format: Patent
• Language: Chinese; English
• Published: 01.01.2021
• Subjects: HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; TESTING

Disclosed herein are immunoassays for detecting an antibody in a biological sample from a subject and/or diagnosing an autoimmune disease in a subject. The disclosed immunoassays use a single, directstep to assess the level of antibody in a biological sample from the subject by simultaneously binding the antibody to a capture antigen (e.g. an unlabeled antigen bound to a solid support) and a labeled antigen not bound to the solid support. 本文公开了用于检测来自受试者的生物样品中的抗体和/或诊断受试者中的自身免疫性疾病的免疫测定法。公开的免疫测定法使用单个、直接步骤来通过使抗体同时结合捕获抗原(例如与固体支持物结合的未标记抗原)和未与固体支持物结合的标记抗原而评价来自受试者的生物样品中的抗体的水平。