Vaccine universal vectors and preparation method and application thereof

• Main Authors: JI MEI, LIU RUITIAN, XIE XIXIU
• Format: Patent
• Language: Chinese; English
• Published: 11.12.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides vaccine universal vectors HBc-S and HBc (1-183) -S which are constructed based on SpyCatcher/SpyTag and take hepatitis B virus core protein as a basis. SpyCatcher is displayed in a main immunodominance region of VLPs and can be subjected to an adhesion reaction with SpyTag at the amino terminal of epitope polypeptide, linear, annular and phosphorylated B cell epitopes SP aredisplayed on the surfaces of VLPs, and a series of HBc-S-P VLPs vaccines are prepared. Mice immunized with the vaccines can generate antibodies specific to the epitopes; and HBc-S-pTau 422 remarkablyimproves cognition and pathological changes of Alzheimer' S disease transgenic mice; HBc (1-183) -S induces Th1 type immunoreaction, and the HBc (1-183) -S-OVA vaccines are of a VLP structure, can activate and promote maturation of DCs, remarkably inhibit growth of tumors and prolong the lifetime of the tumors. 本发明提供了基于SpyCatcher/SpyTag构建的以乙肝病毒核心蛋白为基础的疫苗通用载体HBc-S和HBc(1-183)-S，SpyCatcher展示于VLPs的主要免疫优势区，可与表位多肽氨基端的SpyTag发生黏合