DNA POLYMERASES FOR EFFICIENT AND EFFECTIVE INCORPORATION OF METHYLATED-DNTPS

• Main Authors: BAUER KEITH, ECKERT BARBARA
• Format: Patent
• Language: Chinese; English
• Published: 30.10.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Disclosed are DNA polymerases having improved ability to incorporate methylated-dNTPs, relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases. Further disclosed are kits and reaction mixtures comprising the improved DNA polymerases as well as methods of primer extension using the improved DNA polymerases. 公开了相对于相应的未修饰的聚合酶具有改善的参入甲基化-dNTPs的能力的DNA聚合酶。所述聚合酶可用于多种公开的引物延伸方法中。还公开了相关的组合物，包括重组核酸、载体和宿主细胞，其可用于例如DNA聚合酶的生产。进一步公开了包含所述改进的DNA聚合酶的试剂盒和反应混合物，以及使用所述改进的DNA聚合酶的引物延伸方法。