Detection method for correlation between GARNL1 gene and chicken breed testis traits and application

• Main Authors: ZHU XUENONG, XU JIGUO, TAN YUWEN, ZHOU MIN, GONG JISHANG, WEN ZHENGYA
• Format: Patent
• Language: Chinese; English
• Published: 23.10.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a detection method for correlation between GARNL1 gene and chicken breed testis traits and application, and belongs to the field of genetic engineering. The method comprises the following steps: firstly, extracting genome DNA of a to-be-detected chicken breed, amplifying upstream and downstream 463bp fragments of an rs14532831 site of a 5' regulation region of the GARNL1 gene, performing RFLP typing on an obtained PCR product, and performing statistical analysis on correlation between different genotypes of the rs16492031 site of the GARNL1 gene and cock testis traits.According to the invention, the correlation relationship between the polymorphism of the rs14532831 site of the chicken breed GARNL1 gene and the testis traits of the chicken breed is detected, and the relationship and the method are used as the means for breeding and marking chicken breeds. Compared with existing slaughter breeding, the breeding method is low in cost, easy and convenient to operate and accurate in result