Methods of rescuing stop codons via genetic reassignment with ACE-tRNA
In certain embodiments, the present invention provides a modified transfer RNA (tRNA) comprising a T-arm, a D-arm, and anticodon-arm and an acceptor arm, wherein the T-arm comprises nucleotides that interact with the elongation factor 1 alpha protein, and methods of use thereof. In certain embodimen...
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Format | Patent |
Language | Chinese English |
Published |
25.09.2020
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Abstract | In certain embodiments, the present invention provides a modified transfer RNA (tRNA) comprising a T-arm, a D-arm, and anticodon-arm and an acceptor arm, wherein the T-arm comprises nucleotides that interact with the elongation factor 1 alpha protein, and methods of use thereof. In certain embodiments, the present invention provides a modified transfer RNA (tRNA) comprising a T-arm, a D-arm, and anticodon-arm and an acceptor arm, (a) wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5'-UCA-3' and recognizes TGA stop codons, and wherein the acceptor arm isoperably linked to a arginine, tryptophan or glycine; (b)wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5'-UUA-3' and recognizes TAA stop codons, and whereinthe acceptor arm is operably linked to a glutamine or, glutamate; or (c) wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5'-CUA-3' and recognizes TAG stop codons, and wherein the accep |
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AbstractList | In certain embodiments, the present invention provides a modified transfer RNA (tRNA) comprising a T-arm, a D-arm, and anticodon-arm and an acceptor arm, wherein the T-arm comprises nucleotides that interact with the elongation factor 1 alpha protein, and methods of use thereof. In certain embodiments, the present invention provides a modified transfer RNA (tRNA) comprising a T-arm, a D-arm, and anticodon-arm and an acceptor arm, (a) wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5'-UCA-3' and recognizes TGA stop codons, and wherein the acceptor arm isoperably linked to a arginine, tryptophan or glycine; (b)wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5'-UUA-3' and recognizes TAA stop codons, and whereinthe acceptor arm is operably linked to a glutamine or, glutamate; or (c) wherein the anticodon-arm comprises a tri-nucleotide anticodon, wherein the anticodon is 5'-CUA-3' and recognizes TAG stop codons, and wherein the accep |
Author | AHERN CHRISTOPHER LUECK JOHN D |
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Discipline | Medicine Chemistry Sciences |
DocumentTitleAlternate | 使用ACE-tRNA经由遗传重分配拯救终止密码子的方法 |
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Snippet | In certain embodiments, the present invention provides a modified transfer RNA (tRNA) comprising a T-arm, a D-arm, and anticodon-arm and an acceptor arm,... |
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Title | Methods of rescuing stop codons via genetic reassignment with ACE-tRNA |
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