Gene expression system for probiotic microorganisms

• Main Author: ABBOTT ZACHARY
• Format: Patent
• Language: Chinese; English
• Published: 21.08.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

Provided herein are recombinant microorganisms that express a subject polypeptide. Microorganisms can comprise an expression construct comprising a flagellin promoter operatively linked with a heterologous nucleotide sequence encoding the subject polypeptide. The flagellin promoter sequence can comprise a genetic modification that reduces CsrA inhibition of translation. Microorganisms also can comprise a genetic modification that reduces FlgM inhibition of SigD initiation of transcription. The target polypeptide can be an aldehyde dehydrogenase. Such microorganisms are useful in the treatmentof alcohol hangover. 本文提供了表达主题多肽的重组微生物。微生物可以包含表达构建体，该表达构建体包含与编码主题多肽的异源核苷酸序列可操作地连接的鞭毛蛋白启动子。鞭毛蛋白启动子序列可以包含降低CsrA对翻译的抑制的遗传修饰。微生物还可以包含降低FlgM对SigD转录起始的抑制的遗传修饰。靶多肽可以是醛脱氢酶。这样的微生物可用于治疗酒精宿醉。