Primer composition used for simultaneously detecting multiple feline respiratory tract pathogens, and kit thereof

• Main Authors: XIAO XIANGYU, HAO XIANGQI, LI SHOUJUN, ZHOU PEI
• Format: Patent
• Language: Chinese; English
• Published: 28.07.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of biological detection, and in particular relates to an mPCR primer composition used for simultaneously detecting multiple feline respiratory tract pathogens, and a kit thereof. For establishing an mPCR detection method of feline respiratory tract related pathogens, the invention provides an mPCR primer composition used for detecting feline respiratorytract pathogens, including FHV-F, FHV-R, FeLV-F, FeLV-R, Cf-F and Cf-R; furthermore, according to the mPCR primer composition, an mPCR method used for simultaneously detecting multiple feline respiratory tract pathogens is established; Taq enzyme is used in a detection process; therefore, detection is relatively convenient; the pollution opportunity in a sampling process is reduced; enzyme, ddH O, a premixed primer and a temperature are directly added when detection, so that mPCR is started; the method is superior to other mPCR detection methods; and thus, a high-efficiency, sensitive, specific and low-cost detection